Genomics and bioinformatics laboratory has a wide range of technologies and methodologies including next-generation sequencing (NGS), array CGH, fluorescence-in-situ hybridization (FISH), Karyotyping, HLA-typing, sanger sequencing, high resolution melting curve, as well as routine molecular methodologies such as real-time PCR etc.
NextSeq1000 and NextSeq2000
Applied Biosystems 3500 Genetic Analyzer
Applied Biosystems SeqStudio Genetic Analyzer
COVARIS sonication machine
Light cycler 480
Applied Spectral Imaging (FISH)
Bionano Saphyr Next-Generation Cytogenomics System
We can provide guidance and support with the following:
- Experimental design
- Sample preparation
- Data analysis
The importance of genomic data in medicine has been ever increasing especially in the field of cancer biology and treatment decision for cancer patients. The use of next-generation sequencing (NGS) technologies has been a revolutionizing advancement in providing such information and at a timely manner for use in the clinic.
CSHM scientists are working to provide a high-throughput genomics platform for the biosciences community. This will include support and experimental design and advice, NGS library preparation methods, DNA extraction, long-read sequencing, long mate-pair library construction and exome capture, analysis from transcriptomics to exome sequencing, genome assembly, genome annotation and metagenomics.
NGS can often create large datasets that pose a computational challenge. The Center will provide a genome analytics solution by enabling high-performance computing support.