Overview

Flow Cytometry is a widely used cell biology technology that measures in parallel multiple characteristics of single cells/particles while suspended in a moving fluid stream. It allows rapid quantitative information on numbers, size, granularity. When used in combination with fluorescent dyes or fluorophore-conjugate antibody staining it can provide quantification of cell viability, surface and intracellular/nuclear proteins on particles or live/fixed cells.

FACS stands for fluorescence activated cell sorting. This flow cytometry based-technology allows for simultaneous collection of aforementioned data followed by a sorting step of your cell population of interest.

More information can be found in the links that follow:
https://www.bosterbio.com/protocol-and-troubleshooting/flow-cytometry-principle
https://www.sciencedirect.com/topics/neuroscience/flow-cytometry

Flow Cytometry Facility

CSHM is running a flow cytometry facility equipped with state-of-the-art instruments and is managed by Dr. Chryso Pieridou and Dr. Philippos Demetriou.

We can provide guidance and support with the following:

• Experimental design
• Panel design for multicolour flow cytometry analysis
• Sample preparation for flow cytometry analysis and/or sorting
• Sample acquisition
• Cell sorting
• Data analysis

CELL ANALYSIS

• BD Verse with High Throughput Sampler (HTS) (3 laser system).
• Cytek Aurora with High Throughput Sampler (3 laser system)

 CELL SORTING

• FACSAria III. Up to 11 fluorescent channels available.

For more information,  please contact:
[email protected]
[email protected]