Flow Cytometry Facility

flow cytometry
flow cytometry

Overview

Flow Cytometry is a widely used cell biology technology that measures in parallel multiple characteristics of single cells/particles while suspended in a moving fluid stream. It allows rapid quantitative information on numbers, size, granularity. When used in combination with fluorescent dyes or fluorophore-conjugate antibody staining it can provide quantification of cell viability, surface and intracellular/nuclear proteins on particles or live/fixed cells.

FACS stands for fluorescence activated cell sorting. This flow cytometry based-technology allows for simultaneous collection of aforementioned data followed by a sorting step of your cell population of interest.

More information can be found in the links that follow:
https://www.bosterbio.com/protocol-and-troubleshooting/flow-cytometry-principle
https://www.sciencedirect.com/topics/neuroscience/flow-cytometry

Flow Cytometry Facility

CSHM is running a flow cytometry facility equipped with state-of-the-art instruments and is managed by Dr. Chryso Pieridou and Dr. Philippos Demetriou.

We can provide guidance and support with the following:

  • Experimental design
  • Panel design for multicolour flow cytometry analysis
  • Sample preparation for flow cytometry analysis and/or sorting
  • Sample acquisition
  • Cell sorting
  • Data analysis

CELL ANALYSIS

  • BD Verse with High Throughput Sampler (HTS). Up to

 CELL SORTING

  • FACSAria III. Up to 11 fluorescent channels available.

For more information,  please contact:
[email protected]
[email protected]